The magnetic beads technology has a lot of application fields.
Annexin V-FITC Apoptosis Detection Kits.Garrett, Galloway, Chu, Itell, Stoddard, Wolf, Logue, McDonald, Matsen, Overbaugh: " High resolution profiling of pathways of escape for SARS-CoV-2 spike-binding antibodies." dans: bioRxiv : the preprint server for biology, ( 2020) ( PubMed). Référence (hide) Greaney, Loes, Gentles, Crawford, Starr, Malone, Chu, Bloom: " Antibodies elicited by mRNA-1273 vaccination bind more broadly to the receptor binding domain than do those from SARS-CoV-2 infection." dans: Science translational medicine, ( 2021) ( PubMed).Once the Beads reconstitution, please use it immediately. Upon receipt, please store the Beads at -20☌ for 1 year in lyophilized state. Lyophilized from 0.22 μm filtered solution in PBS, 0.05 % Tween-20, pH 7.4, with 10 % Trehaloseĭo not to freeze thaw the Beads after reconstitution. We provide the SARS-CoV-2 Spike protein RBD-coupled magnetic beads, which could help you to capture the antibody or ACE-2 protein, and easily to follow up with other tests, such as Cell stimulating, biopanning or Flow cytometric analysis. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 Mol/L, the Biotinylated protein can bind to the SA beads irreversibly. The Antigen pre Coupled magnetic beads are Coupled with Biotinylated protein onto streptavidin (SA) magnetic beads. Flow cytometric analysis: This antigen coupled beads can be used as feeder cells (antigen-presenting cells) to run your flow cytometric analysis, but without the hassle of cell culture.More efficient and time saving compared to the plate based capture. Simply use a magnet to separate the bound phages from the unbound ones. In the capturing step, just add the antigen couple beads to your phage library. Biopanning: Biopanning is an affinity selection technique which selects for peptides that binds to a given target.
It can be easily removed using a magnet when its work is done. Cell stimulating: In order to study some downstream mechanisms, this antigen coupled beads can be used to stimulate your target cells.Add your antibody or ACE-2 protein to the beads, incubation and wash the beads.Reconstitute the Beads following the COA, wash the Beads and suspended to a certain concentration by adding assay Buffer.This product is intended for immunocapture, cell stimulating, biopanning and flow cytometry. This ready to use products could greatly save your time and hassle.Ĭoupled amount of S1 protein: 80 μg / 2 mg beadsĬapture ability of Antibody/Sample: > 200 nmol / mg Beads This very first SARS-CoV-2 Spike protein RBD-coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. The pre-coupled magnetic beads coupled with biotinylated SARS-CoV-2 Spike RBD protein to streptavidin conjugated magnetic beads, which can capture the Anti- SARS-CoV-2 antibody or ACE2 protein from cell or serum sample. SARS-CoV-2 Spike protein RBD-coupled magnetic beads RNA Based Therapeutics - Drug Discovery.
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